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Genomes (Grades 11-12)

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Genomes

1. 
The genome of domesticated dogs contains an insulin-like growth factor called IGF-1. This factor is plays a role in determining hte size the dog will be. Which statement about IGF-1 is true?
  1. Large dogs do not produce IGF-1.
  2. Small dogs do not produce IGF-1.
  3. Large dogs have an active allele for IGF-1 and small dogs have a less active allele.
  4. Small dogs lack the IGF-1 allele and large dogs possess it.
2. 
A noncoding strand of 10-base pair DNA was cut three different ways. The first fragment was cut TG, ATG, CCTAG. THe second cut produced AT, GCC, and TACTG fragments. The third cut produced CTG, CTA, and ATGC fragments. What is the correct sequence of the original piece of DNA?
  1. GTCATCCGTA
  2. ATGCCTACTG
  3. ATGACTGCCT
  4. GTCATGTACC
3. 
If the universal adapter sequence is GTCATTGCTTGCAATGTT, which of the following universal primers should be used for DNA sequencing?
  1. GTCATTGCTTGCAATGTT
  2. TTGTAACGTTCGTTACTG
  3. CAGTAACGAACGTTACAA
  4. AACATGCTGGTTAAGTCC
4. 
Which of the following will NOT be included in an open reading frame?
  1. a start codon
  2. a stop codon
  3. coding regions of genes
  4. regulatory sequences
5. 
Which of the following is most likely the genome of a free-living prokaryote?
  1. A genome that is 300 million bp long with over 50 percent repetitive DNA and many introns.
  2. A genome that is 16,000 bp long and arranged in a circle.
  3. A genome that is 3 million bp long and arranged in a single chromosome with little repetitive DNA.
  4. A genome that is 3 million bp long and arranged in a linear chromosome with many introns.
6. 
Which of the following techniques or tools is the best way to determine the minimal genome of a free-living bacterium?
  1. metagenomics
  2. haplotype mapping
  3. bacterial artificial chromosomes
  4. selective inactivation of genes
7. 
Which statement about genomes is true?
  1. Most genes found in the genomes of complex organisms are not required for survival.
  2. As complexity increases the number of genes increases more than the size of the genome.
  3. Prokaryotes develop more chromosomes are they become more complex.
  4. Compartmentalization of eukaryotic cells allows these organisms to have fewer genes than prokaryotes do.
8. 
A                                    found in the dog hookworm is able to move to the mammalian host without using an RNA intermediate.
9. 
In eukaryotic genomes, the highly repetitive sequences called                                                              are not transcribed.
10. 
What are some general characteristics of the human genome, in particular number of base pairs, length of genes, and distribution of those genes?



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