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Recombinant DNA and Biotechnology

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Recombinant DNA and Biotechnology Answer Key

1. 
Which enzyme could be used to join two DNA fragments?
  1. DNA ligase
  2. DNA polymerase
  3. DNA recombinase
  4. DNA endonuclease
2. 
Which statement about recombinant DNA is true?
  1. All restriction endonucleases generate blunt end fragments.
  2. Hydrogen bonds between base pairs are very strong.
  3. Restriction endonucleases cut both strands of DNA at the same time.
  4. Sticky ends prevent DNA ligase activity.
3. 
Why are plant cells, as opposed to animal cells, particularly useful as hosts for recombinant DNA?
  1. Animal cells cannot be cultured.
  2. Plant cells can make totipotent stem cells.
  3. Plant cells have reverse transcriptase.
  4. Plant cells have plasmids.
4. 
What task of DNA recombination does the technique of electroporation complete?
  1. It separates proteins based on size.
  2. It creates antisense DNA.
  3. It measures the expression level of a gene.
  4. It inserts the DNA into the host cell.
5. 
What characteristic makes plasmids useful as a vector?
  1. Many plasmids contain genes that convey antibiotic resistance.
  2. Plasmids do not need to be coaxed to infect cells by artificial means.
  3. Plasmids use the same origin of replication as eukaryotic cells.
  4. Plasmids can accommodate a large amount of DNA.
6. 
The enzyme responsible for the synthesis of cDNA from mRNA is called reverse transcriptase.
7. 
Hosts that are altered by the insertion of recombinant DNA are known as transgenic organisms.
8. 
Which property distinguishes an expression vector from a typical vector?
  1. possession of a recognition sequence for a restriction enzyme
  2. presence of reverse transcriptase
  3. a promoter sequence
  4. ability to replicate independently within the host
9. 
Biotechnology and the use of genetically modified organisms for food is a very controversial topic. Why are people afraid to consume the genetically enhanced crops?
  • Genetically modified organisms have many unanswered questions attached to them. People fear the unknown and worry that if they consume these GMOs then they will be eating food that contains transgenes. Also, they worry that the crops, which are grown outside will transfer the transgenes to other organisms. This could cause beneficial insects to be harmed or the beneficial traits could transfer to weeds. While there is no right answer, GMOs are something that science, medicine, and the general population need to be aware of.
10. 
If all factors are equal, which genetically altered food would be of the most concern to human health?
  1. food that contains an inserted gene that allows the plants to grow faster
  2. food that contains an inserted gene that produces beta-carotene in beans
  3. food that contains an inserted gene that promotes flowering
  4. food that contains an inserted gene that allows plants to grow in soil with a high salt concentration
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